Photosynthesis Light Reactions Simulator: Electron Transport Chain

simulator advanced ~15 min
Loading simulation...
ETR = 168 μmol e⁻/m²/s — healthy C3 plant in moderate light

At 500 μmol/m²/s PAR, 25°C, 420 ppm CO₂, and mild water stress, the electron transport rate is approximately 168 μmol/m²/s, producing ATP and NADPH to drive carbon fixation.

Formula

ETR = PAR × f_PSII × α_leaf × Φ_PSII
A_net = ETR/4 - R_d - Φ × O₂/CO₂ (net assimilation)
Φ_PSII = (F_m' - F_s) / F_m' (PSII operating efficiency)

Harvesting Sunlight

Every oxygen molecule you breathe was produced by the light reactions of photosynthesis. In thylakoid membranes of chloroplasts, an extraordinary molecular machine captures photons, splits water, and channels electrons through a series of protein complexes to produce the ATP and NADPH that power carbon fixation. This electron transport chain is the engine of virtually all life on Earth.

The Z-Scheme of Electron Transport

Electrons follow a Z-shaped energy path through two photosystems. Photosystem II (P680) absorbs light and uses that energy to extract electrons from water, releasing O₂ as a byproduct. These electrons pass through the cytochrome b6f complex (generating a proton gradient for ATP synthesis) to Photosystem I (P700), which absorbs a second photon and reduces ferredoxin, ultimately producing NADPH. The two-photon requirement explains why the quantum yield of photosynthesis is roughly 0.125 (one O₂ per 8 photons).

Light Response and Saturation

At low light, photosynthetic rate increases linearly with irradiance — each additional photon contributes to electron transport. Above the light saturation point, downstream carbon fixation becomes limiting, and excess absorbed energy must be safely dissipated. Plants use non-photochemical quenching (NPQ) — converting chlorophyll excited states to heat via the xanthophyll cycle — to protect PSII from photodamage. This simulation models the light response curve and how stress factors shift it.

Environmental Regulation

Temperature, CO₂ concentration, and water availability all modulate light reaction efficiency. Heat destabilizes the oxygen-evolving complex of PSII. Drought causes stomatal closure, reducing CO₂ supply and backing up the electron transport chain. Low CO₂ increases photorespiration, wasting energy. Understanding these interactions is crucial for predicting crop productivity under climate change — and this simulator lets you explore each factor independently.

FAQ

What are the light reactions of photosynthesis?

The light reactions occur in thylakoid membranes and convert light energy into chemical energy (ATP and NADPH). Photosystem II splits water and releases O₂, passing electrons through cytochrome b6f to Photosystem I, which reduces NADP⁺ to NADPH. The proton gradient drives ATP synthase.

What is the Z-scheme?

The Z-scheme describes the path of electrons through the light reactions, plotted as redox potential versus progress. Electrons are boosted twice — once by PSII (P680) and once by PSI (P700) — creating a Z-shaped energy diagram from water (+0.82 V) to NADPH (-0.32 V).

What is photoinhibition?

Photoinhibition is the light-induced decrease in photosynthetic efficiency caused by damage to PSII reaction centers. It occurs when light absorption exceeds the capacity for electron transport, generating reactive oxygen species that damage the D1 protein. Plants protect themselves through non-photochemical quenching and the xanthophyll cycle.

How does electron transport rate relate to carbon fixation?

The light reactions produce ATP and NADPH consumed by the Calvin cycle. Four electrons must flow through the chain to fix one CO₂: 3 ATP and 2 NADPH per CO₂. Thus, the electron transport rate sets the upper limit on carbon fixation rate.

Sources

Embed

<iframe src="https://homo-deus.com/lab/photochemistry/photosynthesis-light/embed" width="100%" height="400" frameborder="0"></iframe>
View source on GitHub