Membrane Transport: Nernst & Goldman Equations Visualized

simulator intermediate ~10 min
Loading simulation...
Vm = -72.5 mV — near K+ Nernst potential

With standard ionic concentrations and a K+/Na+ permeability ratio of 25:1, the Goldman equation yields a resting potential of -72.5 mV, close to the K+ Nernst potential of -89.1 mV.

Formula

E_ion = (RT / zF) × ln([ion]_out / [ion]_in)
Vm = (RT/F) × ln((P_K[K+]out + P_Na[Na+]out) / (P_K[K+]in + P_Na[Na+]in))

Ions and Membranes

Every living cell is enclosed by a lipid bilayer that is selectively permeable to ions. Potassium, sodium, calcium, and chloride ions are maintained at vastly different concentrations inside and outside the cell by ATP-powered pumps. This concentration gradient is a form of stored energy — a biological battery. The Nernst equation quantifies the voltage that balances a single ion's concentration gradient, while the Goldman equation integrates contributions from all permeable ions.

The Nernst Potential

For potassium with typical mammalian concentrations (140 mM inside, 5 mM outside), the Nernst potential is approximately -89 mV. This means that at -89 mV, K+ ions are in electrochemical equilibrium — no net flow occurs. For sodium (12 mM inside, 145 mM outside), the Nernst potential is about +67 mV. The cell's actual resting potential lies between these extremes, weighted by relative permeabilities.

Goldman Equation and Resting Potential

The Goldman-Hodgkin-Katz voltage equation accounts for multiple ion species simultaneously. At rest, K+ permeability dominates, pulling the membrane potential toward -90 mV. During excitation, Na+ permeability surges, driving the potential toward +60 mV. This simulation lets you adjust permeability ratios and concentrations to see how the resting potential shifts in real time.

Clinical Relevance

Disruptions in ionic balance cause serious pathology. Hyperkalemia (high extracellular K+) depolarizes cardiac cells and can trigger fatal arrhythmias. Channelopathies — genetic defects in ion channels — underlie epilepsy, long QT syndrome, and cystic fibrosis. Understanding membrane transport physics is fundamental to pharmacology, cardiology, and neuroscience.

FAQ

What is the Nernst equation?

The Nernst equation calculates the equilibrium potential for a single ion species: E = (RT/zF) × ln([ion]out/[ion]in). At this voltage, the electrical force exactly balances the concentration gradient, producing zero net flux for that ion.

How does the Goldman equation differ from Nernst?

The Goldman-Hodgkin-Katz equation extends Nernst to multiple ion species, weighting each by membrane permeability. It predicts the actual resting membrane potential, which lies between the Nernst potentials of the permeable ions.

Why is the resting potential close to E_K?

At rest, the membrane is roughly 25-100 times more permeable to K+ than Na+. Since K+ dominates the permeability, the Goldman equation yields a potential close to the K+ Nernst potential (~-90 mV) rather than the Na+ Nernst potential (+60 mV).

What happens during an action potential?

During an action potential, voltage-gated Na+ channels open, dramatically increasing Na+ permeability. The Goldman potential shifts toward E_Na (+60 mV), causing rapid depolarization. K+ channels then open to restore the resting potential.

Sources

Embed

<iframe src="https://homo-deus.com/lab/biophysics/membrane-transport/embed" width="100%" height="400" frameborder="0"></iframe>
View source on GitHub